2×KunGre™ PCR Master Mix (With Dye)
Introduction
This product contains high-purity Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer, and stabilizer at a concentration of 2 ×. It has the advantages of fast and simple, high sensitivity, strong specificity and good stability. It can minimize human error, and can be used for high specific PCR reaction and high GC content (> 60%) with secondary structure and other complex template amplification and large-scale gene detection. The PCR product has a prominent "A" base attached to the 3 'end, which can be directly used for TA vector cloning after purification. This product contains blue dye. After the PCR reaction is completed, you can directly load the sample for electrophoresis without adding a loading buffer; it can also be purified for subsequent operations such as enzyme digestion, ligation, and fluorescent sequencing. Blue dye indicates the progress of electrophoresis, and its migration speed is similar to that of 500 bp double-stranded DNA fragment in 1% TAE agarose gel.
Components
Cat. No. | Description | Quantity |
GT101 | 2×KunGre ™ PCR Master Mix (With Dye) | 1 ml |
Note: 2×KunGre ™ PCR Master Mix (With Dye) contains Taq DNA Polymerase, 300 μM dNTP each, 20 mM Tris-HCl (pH8.7), 100 mM KCl, 3 mM MgCl2.
Shipping and Storage
Keep at -20°C for long-term storage. 2×KunGre ™ PCR Master Mix (With Dye) are stable at 4°C for six months. For daily use, we recommend keeping an aliquot at 4°C.
Applications
1) Gene test, half- QPCR and detection of trace DNA.
2) Gene cloning, SNP analysis and site-directed mutagenesis.